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A Beginner’s Guide to Potting Up Seedlings

Two hands packing a young eggplant seedling into a larger pot

A Beginner’s Guide to Potting Up Seedlings

There’s something deeply rewarding about nurturing a tiny seed into a thriving plant. But when a plant outgrows its container, potting up can seem daunting. Fortunately, with care and a couple of considerations, potting up is easy and will help to keep your plants happy!

In this article, we will explain the benefits of potting up and how we do so here at Heritage Farm. We will also show you when and how to pot up your seedlings, and give you some helpful tips. Whether you’re a first-time gardener or brushing up on your green thumb skills, this guide will help you set your plant starts up for success.

What is “potting up”?

Two hands placing an asparagus seedling into a larger pot
Potting up asparagus plant starts at Heritage Farm

Potting up is the process of moving a plant up to a larger container it needs more space to grow.

It is a common practice for crops that have an extended germination period or spend a long time growing in the greenhouse, such as:

Why should I pot up my seedlings?

Simply put, potting up seedlings will allow them to grow into large, healthy, and happy plants. Caring for indoor seedlings is a delicate process, and young plants are sensitive to their environment. 

As a seedling grows bigger and occupies more and more space within its pot, it can begin to get crowded. Crowding creates various problems for the plant:

  1. It depletes the nutrients available to the plant through the soil. 
  2. It causes the soil to dry out faster and need more frequent watering.
  3. It can stunt the growth of the seedling.
  4. It can cause the seedling to grow leggy and weak.

Giving your seedlings a larger container if they need one reduces stress. This helps them grow strong roots and stems. It also encourages the plants to grow bushier rather than taller.

Small, feathery asparagus plant starts in a 200-cell tray
Sowing asparagus in 200-cell trays helps save space and resources
A row of asparagus plants in a greenhouse
Potting up allows these asparagus plant starts to grow big, strong, and healthy.

Why not sow seeds in a larger container to begin with?

If you garden on a small scale and aren’t worried about running out of indoor space, starting seeds in larger containers is perfectly okay! However, there are risks with sowing seeds into pots that are too large.

When starting seeds indoors, it’s important for the size of the seedling to roughly match the size of its pot. This has to do with water management. As you water the seedlings, the water saturates the soil. The water that soil absorbs leaves in two ways: evaporation or transpiration by the plant.

If a seedling is too small for its container, its tiny roots can only absorb a little bit of the water. Most of the soil in the pot stays saturated and will not dry out. This can lead to root rot, yellowing leaves, stunted growth, mold, and disease.

Potting up at Heritage Farm

On a larger scale, potting up helps with space and resource management. Here at Heritage Farm, we grow thousands of seedlings in our greenhouses each spring. At this large scale, we need to plan our greenhouse space efficiently to avoid running out of room.

The farm team plants many crops, such as tomatoes, peppers, flowers, and herbs in 200-cell trays. One 200-cell tray occupies the same amount of greenhouse space as just 32 3-inch pots.

Tomato seedlings in a 200-cell tray
Tomato seedlings in a 200-cell tray
Several large trays holing many 3" plastic pots, each with a tomato seedling
Growing tomatoes in larger pots takes up significantly more space

Starting seeds in small pots also helps to mitigate the challenges of low germination. If a tomato variety has low germination and some seeds don’t germinate, this wastes the space and resources used to start those seeds.

When growing tomatoes in 200-cell trays, an instance of low germination might only waste a few square inches of greenhouse space. If these tomato seeds were planted in 3-inch pots, a few square inches would turn into significantly more wasted space, soil, and water.

A 20-row tray of tomato seedlings in a greenhouse, with a bare patch of soil in the middle
This case of low germination only wasted a few square inches of greenhouse space.

Another option, as opposed to seeding in individual cells, is to broadcast seeds into open flats or sow in small trenches. For example, in 2025 we grew 1,300 tomato plants and sowed the tomato seeds in 20-row trays. These trays each have 20 small rows, which can each fit 20-30 tomato seeds. A whole 20-row tray can therefore fit upwards of 600 tomato seedlings. 

8 plant trays, each with 20 rows full of tomato seedlings, arranged in a greenhouse
These tomato seedlings were planted in trenches in 20-row trays.

A tray of the same size can only fit 32 3-inch pots. The same 600 tomato seedlings in 3-inch pots would need upwards of 19 trays, and a whole lot of greenhouse space. 

When should I pot up my seedlings?

Look for these signals to know when to pot up your plants:

  • True leaves
  • Emerging roots
  • Holding soil shape
A drawing of a small seedling with its leaf, cotyledon, and hypocotyl labeled.
Pot up seedlings once they grow their first pair of true leaves.

True leaves: An easy way to tell whether a seedling is ready for potting up is to look for true leaves. When a seedling germinates, it produces its first set of leaves, called cotyledons, from the material within the seed. These leaves often look quite different from the leaves that the plant continues to grow.

The second set of leaves that the plant produces is the first of its “true” leaves. The plant will grow true leaves for the rest of its life. Once a seedling has at least one or two sets of true leaves, it can handle the stress of potting up.

A small tomato plant with one set of cotyledon leaves and one smaller set of emerging true leaves.
This tomato seedling is starting to sprout its first set of true leaves.

Emerging roots: Look beneath the pot. When a plant outgrows its pot, often (but not always) the plant’s roots will begin to grow out of the pot’s drainage holes. If you see any root growth outside the pot, the plant needs a larger space to grow.

Holding shape: Carefully remove the seedling from its pot. The seedling’s roots should hold the shape of the media well, forming a root ball. This indicates strong root growth and that the plant can handle the potting-up process.

A hand holding up a small pepper seedling with the roots exposed
A plant start is ready to pot up when the roots have filled the container and can hold the shape of the soil.

Watch this video to learn how to identify cotyledons and true leaves:

How to pot up seedlings

Step 1: Water your seedlings in the morning of the day you plan to pot up, saturating the seedlings’ roots and keeping the root ball moist. Saturated roots help mitigate the shock of transplanting. 

Make sure the aerial parts of the plant (such as the stem and leaves) are dry to help prevent damage when handling.

Many pepper seedlings in a 200-cell tray, ready for potting up
Water the seedlings in the morning of the day you pot up.

Step 2: Gather your new containers and some potting mix. Make sure your containers of choice have drainage holes. 3″ plastic greenhouse pots work great.

Fill the new containers with soil. Your soil mix should be rich in compost or other organic matter to give the seedling an extra boost of nutrients. The soil mix should also be damp, but not too wet, and should not drip when squeezed.

A 200-cell tray of pepper seedlings next to an empty tray of larger pots with soil
Gather your plant starts, new containers, and soil.

Step 3: Create holes in the centers large enough to fit the entire root ball of the plant.

Several 3" greenhouse pots filled with soil with large holes in the middle
Create holes big enough for the root ball to fit into

Step 4: Carefully remove the seedlings from their old pots. If you have only one seedling per pot, it is unnecessary to disturb the roots unless the plant is very root-bound.

If you have more than one seedling per pot, gently tease apart their roots and separate the plants. When teasing plants apart, some roots will break, and you may hear some pops and tears. These sounds may alarm gardeners, but as long as the seedlings have their main roots attached to the stem, they will be fine.

Two hands teasing apart one small tomato seedlings from two others
If you have more than one seedling per pot, tease apart the seedlings before potting up

Step 5: Carefully place the seedling into the divot in its new pot and cover its roots with soil.

Two hands packing a young eggplant seedling into a larger pot
Place the entire plant start in the hole in the new pot.

Gently pack the soil around the base of the plant, but don’t be afraid to apply a little pressure. This helps prevent air bubbles beneath the surface, ensuring good soil-to-root contact.

A note on planting depth:

Tomatoes, like eggplants and peppers, benefit when planted deeper than the soil line. Along a tomato seedling’s stem are tiny nodes that have the potential to develop into adventitious roots. Plant the tomato seedlings deep enough so that the soil reaches just beneath its cotyledons. 

A three inch pot with a tomato seedling. The tomato seedling has much of its stem above the soil line.
This tomato seedling is not planted deep enough.
A three-inch pot with a small tomato seedling. The seedling's stem is buried to just beneath its first set of leaves.
Plant tomato seedlings deep enough that the soil reaches just below its cotyledons.

Most food crop seedlings with stems can handle having part of their stem buried. 

Be careful not to plant the seedling too deep. Avoid getting any soil on top of the growing point of the plant, from which the plant will continue to produce new leaves. At the tender growing point of the plant, soil can damage the leaves and invite disease. 

Step 5: Immediately water the plant starts in their new containers to saturate the new soil. This will alleviate stress for the newly transplanted seedling.

A person using a hose to water many trays of plant starts in a greenhouse
Water your plant starts after potting up

Instructional Videos

Watch the following instructional videos to learn how to pot up:

  1. How to Pot Up Asparagus Seedlings
  2. How to Put Up Pepper Seedlings